Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana.
Identifieur interne : 001672 ( Main/Exploration ); précédent : 001671; suivant : 001673Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana.
Auteurs : Joe Win [Royaume-Uni] ; Sophien Kamoun ; Alexandra M E. JonesSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Chromatographie en phase liquide (méthodes), Immunité des plantes (MeSH), Immunoprécipitation (méthodes), Phytophthora (génétique), Phytophthora (pathogénicité), Spectrométrie de masse en tandem (méthodes), Tabac (génétique), Tabac (microbiologie), Tabac (métabolisme), Techniques de transfert de gènes (MeSH).
- MESH :
- génétique : Agrobacterium tumefaciens, Phytophthora, Tabac.
- microbiologie : Tabac.
- métabolisme : Tabac.
- méthodes : Chromatographie en phase liquide, Immunoprécipitation, Spectrométrie de masse en tandem.
- pathogénicité : Phytophthora.
- Immunité des plantes, Techniques de transfert de gènes.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Chromatography, Liquid (methods), Gene Transfer Techniques (MeSH), Immunoprecipitation (methods), Phytophthora (genetics), Phytophthora (pathogenicity), Plant Immunity (MeSH), Tandem Mass Spectrometry (methods), Tobacco (genetics), Tobacco (metabolism), Tobacco (microbiology).
- MESH :
- genetics : Agrobacterium tumefaciens, Phytophthora, Tobacco.
- metabolism : Tobacco.
- methods : Chromatography, Liquid, Immunoprecipitation, Tandem Mass Spectrometry.
- microbiology : Tobacco.
- pathogenicity : Phytophthora.
- Gene Transfer Techniques, Plant Immunity.
Abstract
Effectors of plant pathogens play important roles in not only pathogenesis but also plant immunity. Plant pathogens use these effectors to manipulate host cells for colonization, and their activities likely influence the evolution of plant immune responses. Analyses of genome sequences revealed that oomycete pathogens, such as Phytophthora spp., possess hundreds of RXLR effectors that are thought to be delivered into the host cells and hence function inside the cells by interacting with the host protein complexes. This article describes a co-immunoprecipitation protocol aimed at identifying putative target complexes of the effectors by transiently overexpressing the tagged effectors in planta. The identification of the eluted protein complexes was achieved by LC-MS/MS mass spectrometry and peptide spectrum matching.
DOI: 10.1007/978-1-61737-998-7_15
PubMed: 21359809
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Jones</name><name sortKey="Kamoun, Sophien" sort="Kamoun, Sophien" uniqKey="Kamoun S" first="Sophien" last="Kamoun">Sophien Kamoun</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Win, Joe" sort="Win, Joe" uniqKey="Win J" first="Joe" last="Win">Joe Win</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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